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Transmission Electron Microscopy Laboratory

Summary:

The TEM laboratory provides access to ultrastructural analysis of biological specimens via a Jeol 100 CX transmission electron microscope. The TEM is equipped with AMT 4 MP digital camera to facilitate image acquisition and 3D reconstruction from serial sections. In addition, to conventional EM procedures of in situ and cell culture specimens the laboratory also offers imunnogold labeling and the visualization of fluorescent dyes after photoconversion.

Resources:

Biological Specimens

Instruments

  • JEOL 100CX Transmission Electron Microscope ( Transmission electron microscope )

  • Leica EM UC6 ultramicrotome ( Ultratome )

    "The Leica EM UC6 features a Touch Screen Control Panel or a Keypad Control Panel.
    Both options enable fast and safer alignment of knife and specimen, and the programmable knife and cutting movements make trimming easy. With Leica’s AutoTrim, you can automatically trim a specimen to a predetermined level in the block face, ideal for morphometric studies. Three dependent, built-in, brightness- controlled LED light sources provide outstanding illumination for top light, back light, and specimen transillumination."

  • Pelco BioWave ( Microwave tissue processor )

Services

Software

  • Adobe Photoshop ( Software )

  • Fiji software ( Software )

    "Fiji is an image processing package. It can be described as a distribution of ImageJ (and soon ImageJ2) together with Java, Java 3D and a lot of plugins organized into a coherent menu structure. Fiji compares to ImageJ as Ubuntu compares to Linux.
    The main focus of Fiji is to assist research in life sciences.
    For users, Fiji is easy to install and has an automatic update function, bundles a lot of plugins and offers comprehensive documentation.
    For developers, Fiji is an open source project hosted in a Git version control repository, with access to the source code of all internals, libraries and plugins, and eases the development and scripting of plugins."

  • Image J ( Software )


Web Links:

Last updated: 2017-05-01T09:41:58.356-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016